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b cell surface markers  (Miltenyi Biotec)


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    Miltenyi Biotec b cell surface markers
    B Cell Surface Markers, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b+cell+surface+markers/pm37995656-725-16-12?v=Miltenyi+Biotec
    Average 95 stars, based on 7 article reviews
    b cell surface markers - by Bioz Stars, 2026-07
    95/100 stars

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    Binding analysis of recombinant SNAP-TTC-BG647 on polyclonally activated CD19 + B cells. CD19 + B cells were isolated from leukocyte filters by density gradient centrifugation and magnetic cell separation with anti-CD19 beads. Isolated B cells were activated by adding 2.5 μg/ml CpG and 50 ng/ml IL-21 to the cell culture medium. On day 4 of cultivation, the cells were analyzed by flow cytometry. Surface a and intracellular b staining with the recombinant SNAP-TTC-BG 647 (1:25) was performed

    Journal: BMC Biotechnology

    Article Title: Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen

    doi: 10.1186/s12896-016-0249-x

    Figure Lengend Snippet: Binding analysis of recombinant SNAP-TTC-BG647 on polyclonally activated CD19 + B cells. CD19 + B cells were isolated from leukocyte filters by density gradient centrifugation and magnetic cell separation with anti-CD19 beads. Isolated B cells were activated by adding 2.5 μg/ml CpG and 50 ng/ml IL-21 to the cell culture medium. On day 4 of cultivation, the cells were analyzed by flow cytometry. Surface a and intracellular b staining with the recombinant SNAP-TTC-BG 647 (1:25) was performed

    Article Snippet: The staining was performed with the following monoclonal antibodies against the human B cell specific surface markers CD19 (Becton Dickinson; Catalogue number: 560911), CD27 (Becton Dickinson; Catalogue number: 337169), CD38 (Becton Dickinson, Catalogue number: 345806) and biotinylated IgD (Becton Dickinson; Catalogue number: 555777) in combination with streptavidin Alexa-Fluor 680 (Life Technologies; Catalogue number: S21378) in combination with the recombinant TTC protein followed by an Alexa Fluor 488-conjugated anti-penta-his antibody (Qiagen; Catalogue number: 35310).

    Techniques: Binding Assay, Recombinant, Isolation, Gradient Centrifugation, Magnetic Cell Separation, Cell Culture, Flow Cytometry, Staining